Protein-DNA Interaction Analysis Brochure. Dounce Homogenizer (Tissue Grinder) Each glass dounce homogenizer is provided with the dounce shaft and two pestles. One pestle has a tight fit within the shaft of the dounce, while the other pestle has a looser fit. Figure 1: Image of Dounce Homogenizer containing the shaft and two pestles.
The 2010 Geno/Grinder® offers analysts a versatile high-throughput tissue homogenizer for plant or animal tissue homogenization. The patented design with a true linear grinding motion provides the most efficient bead milling mechanism for tissue homogenization and cell lysis for extraction of DNA, RNA, proteins or enzymes.
FastPrep®-24 Instrument lyses thoroughly and quickly any tissues and cells and thus allows easy and reproducible isolation of stable RNA, active proteins and full-length genomic DNA. Base Catalog Number. 6004500. Dimensions. Height 465 mm; Oval Base 437 x 332 mm.
The microHomogenizer™ tissue grinders can be used in preparation for any downstream application you prefer and can be bought with or without lysing media. Literature and Resources: Protocols: microHomogenizer™ - Tissue Homogenization Protocol Additional Information:
The FastPrep-24™ Classic uses a unique, optimized motion to disrupt cells through the multidirectional, simultaneous beating of specialized Lysing Matrix beads on the sample material. Developed for difficult and resistant samples, the FastPrep …
The FastPrep-24™ Classic uses a unique, optimized motion to disrupt cells through the multidirectional, simultaneous beating of specialized Lysing Matrix beads on the sample material. Developed for difficult and resistant samples, the FastPrep-24™ Classic lyses thoroughly and quickly any tissues and cells and thus allows easy and reproducible isolation of stable RNA, …
…lysis, grinding or homogenization Faster and more effective than tissue grinders Mix 1, 2 or 3 tubes simultaneously Extremely compact, less than 7" wide Eliminates the cross contamination associated with hand held homogenizers Compatible with these Beads and Prefilled Kits…
The high-performance characteristics of the Bertin Instruments homogenizers, together with a wide variety of lysing and grinding kits, offer excellent reproducibility and a great degree of flexibility in a broad range of research areas including microbiology, pharmacology, cancer, forensic medicine, the environment, and even agronomy.
Precellys Lysing Kits are specifically designed by Bertin Instruments to be used with Precellys tissue homogenizers. After optimized tissue lysis, the extraction of nucleic acids (DNA extraction, RNA extraction) is perfected using the DNA- and RNA-free guarantee of Precellys lysing kits. Watch the other videos.
BioEcho Tissue Grinding Pestles are disposable pestles for facilitated lysis of plant or animal tissues by grinding. The pestle tip precisely fits the bottom of a 1.5 ml reaction tube and allows efficient mechanical disruption of plant and animal …
• Pestle and tube homogenizer (also called tissue grinder) DUALL. P ® P, All-Glass. Kontes. o Choose the homogenizer article according to the volume of medium required, e.g. the article No. 885450-0022 can be used to grind tissue in 5 ml of lysis buffer. • Falcon. tubes: BD Falcon #352097, polypropylene (Fisher Cat. #14-959-70C). • Eppendorf
Several methods are commonly used to physically lyse cells to extract proteins, including mechanical disruption, liquid homogenization, high frequency sound waves (sonication), freeze/thaw cycles, and manual grinding. The choice of cell lysis method depends on the type of cells, volume, and sensitivity of proteins being extracted.
Precellys® MK28 Lysing Kit, Hard Tissue Grinding, Bertin. The custom designed reinforced tubes are intended for bead beating applications, and feature O-Rings and reinforced materials for dry grinding valuable or very hard samples. It is designed for hard tissue grinding like muscle, skin, spincor, bone, teeth, hair, seeds, corn, rice and more.
Tissue Grinder Kontes VWR catalog #KT885000-0002 Component Storage Bio-Plex phosphoprotein and total target assays Coupled beads (50x) Store at 4ºC in the dark. Do not freeze. Detection antibodies (25x) Store at 4ºC. Lysates (p cer at io n = 20 µg/ml) Store at –20ºC. Lysates can be frozen n ha wd up o 5t ime s. Bio-Plex cell lysis kit
used to maintain tissue samples at subzero temperatures during grinding to increase yields of RNA, proteins and ... Add 500 microliters of lysis buffer to tube (or as instructed by method). 3. ... A full range of accessories are available for the Geno/Grinder from different sized titer plates, vials and vial sets to different types of
The Geno/Grinder® is specifically designed for vigorous up-and-down shaking of deep well titer plates or sample tubes up to 50mL. It provides an efficient m...
Cryogenic grinding is useful as a first step in preparing samples for chemical lysis or subsequent mechanical processing. Its true value is that samples can be reduced from large solid items to small particles without tremendous input of heat. With smaller particle size, the sample can be rapidly dissolved, as is done for RNA isolation.
The TissueLyser II can also disrupt large samples when used in combination with Grinding Jar Sets. Applications The ability to process up to 192 samples per run makes the TissueLyser II the ideal front-end solution to access biological information for genomics, transcriptomics, and proteomics applications.
Tissue grinding CKMix50. 7mL kit includes 50 preps of a mix of ceramic (zirconium oxide) mix beads of 2.8mm and 5.0mm in 7mL standard tubes with O-ring. 15mL kit includes 25 preps of a mix of ceramic (zirconium oxide) mix beads of 2.8mm and 5.0mm in 15mL standard tubes.CKMix50 is designed for grinding whole organs.>> Visit the product website
The Precellys® range, fully safe, versatile tissue homogenization The Precellys ® range allows you to prepare samples in full safety and without cross contamination, for both routine applications and R&D projects. • For infrequent use, the Minilys® homogenizer allows you to prepare up to three samples at the same time.
Precellys® MK28 Lysing Kit, Hard Tissue Grinding, Bertin. The custom designed reinforced tubes are intended for bead beating applications, and feature O-Rings and reinforced materials for dry grinding valuable or very hard samples. It is …
Cell or tissue disruption methods. Many cell lysis methods have been developed to obtain the best possible yield and purity for different species of organisms, sample types (cells or tissue), and target molecule or subcellular structure. Cell lysis methods can be divided into two main categories, reagent-based and physical disruption.
Shop Smart & Save: Pyrex® Tissue Grinders for Cell Culture Homogenization, Corning® Javascript is disabled on your browser. To view this site, you must enable JavaScript or upgrade to a JavaScript-capable browser.
CKMix50-R is designed for wet or dry tissue grinding like hair, whole piece of mouse lung, heart or dry hair, mouse bones and more. Sample size range:from 20mg to 200mg tissue or 200microL to 1.6mL culture. Compatible with Precellys Evolution, Precellys 24, Cryolys and Minilys. MORE INFORMATION. - Download the brochure. - Request a free demo kit.
Sample Grinding Kit is designed to disrupt cell or tissue samples. It utilizes an abrasive grinding resin and grinding pestle to rupture cells for protein extraction. Intracellular organelles are also disrupted, resulting in the liberation and extraction of all proteins soluble in the extraction solution.
Physical disruption methods of cell lysis. Mechanical disruption Mechanical methods rely on the use of rotating blades to grind and disperse large amounts of complex tissue, such as liver or muscle. The Waring blender and the Polytron are commonly used for this purpose.
Traditional Methods of Cell Lysis for Protein Extraction. Several methods are commonly used to physically lyse cells to extract proteins, including mechanical disruption, liquid homogenization, high frequency sound waves (sonication), freeze/thaw cycles, and manual grinding. The choice of cell lysis method depends on the type of cells, volume ...